کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5768890 1628521 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Heat and/or ultrasound pretreatments motivated enzymolysis of corn gluten meal: Hydrolysis kinetics and protein structure
ترجمه فارسی عنوان
پیشگیری از گرما و / یا اولتراسوند باعث تحریک آنزیمیولیز غلات گلوتن ذرت: سینتیک هیدرولیز و ساختار پروتئین
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


- Heat/ultrasound pretreatment on enzymolysis of corn gluten meal protein was investigated.
- Best hydrolysis rate and degree was in sonicated/preheated corn gluten meal protein.
- Structure and morphology of heat/ultrasound pretreated corn protein was changed.

The purpose of this study was to investigate the effect of pretreatments of heat (HP), ultrasound (UP), and combinations of heat/ultrasound (HPUP) and ultrasound/heat (UPHP) on protamex-catalyzed enzymolysis of corn gluten meal (CGM). Enzymolysis kinetics, molecular conformation and microstructure of CGM protein were monitored. Enzymolysis reaction rate increased with increasing substrate concentration and 80 g/L resulted in the highest hydrolysis reaction rate. The best improvement in CGM enzymolysis was obtained by HPUP (KM value decreased by 11.56%) and then followed by UPHP (KM value decreased by 9.97%). Fluorescence spectra and fourier transform infrared (FTIR) spectra indicated that the CGM pretreatments induced molecular unfolding, exposing the hydrophobic groups. HPUP and UPHP pretreatments both decreased the α-helix content by 12.2% and increased the random coil content by 2.23 and 2.60%, respectively. Scanning electron microscopy and atomic force microscopy showed that HPUP and UPHP changed surface topography and distribution of protein particles, enhancing the rate of enzymolysis. In conclusion, combined ultrasound and heat pretreatment and controlled enzymolysis could be an effective method for the functionality modification of globular proteins.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 77, April 2017, Pages 488-496
نویسندگان
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