کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5769682 | 1628781 | 2017 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rescue and in vitro culture of herbaceous peony immature embryos by organogenesis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Herbaceous peony (Paeonia lactiflora Pall.) is a landscaping plant that has considerable development prospects worldwide. However, its embryo dysplasia severely limits the breeding of high-quality P. lactiflora cultivars by the cross-breeding method. In this study, the immature natural hybrid embryos of P. lactiflora 'Zi Fengyu' with double flower were used to study its rescue and in vitro culture by organogenesis. The results showed that the best medium for adventitious bud induction was modified Murashige and Skoog (MS) medium supplemented with 1.0Â mg/L thidiazuron (TDZ), 600Â mg/L acidic hydrolysis casein (ACH), 10% coconut water and 100Â g/L sucrose. The best medium for adventitious bud multiplication was modified MS medium supplemented with 4.0Â mg/L TDZ, 15% coconut water, 60Â g/L sucrose and 0.1% activated charcoal (AC). Modified MS medium containing 0.5Â mg/L gibberellic acid (GA3), 100Â g/L sucrose and 0.1% AC was the more suitable medium for adventitious bud growth. For root regeneration, indole-3-acetic acid (IAA) was a more effective plant growth regulator (PGR), and 1/2 MS medium supplemented with 0.5Â mg/L IAA, 30Â g/L sucrose and 0.1% AC was the more suitable medium, resulting in a rooting rate of up to 92.12%, and the survival rate was 70.8%. Additionally, histological observation confirmed that the rescue and in vitro culture of P. lactiflora immature embryos by organogenesis was successful. These results provide useful technical support for breeding high-quality P. lactiflora cultivars by the cross-breeding method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 217, 15 March 2017, Pages 123-129
Journal: Scientia Horticulturae - Volume 217, 15 March 2017, Pages 123-129
نویسندگان
Daqiu Zhao, Yinfang Xue, Min Shi, Jun Tao,