Insights into potentially toxic effects of 4-aminoantipyrine on the antioxidant enzyme copper-zinc superoxide dismutase

- This work established the binding mode of AAP with Cu/ZnSOD on molecular level.
- The mechanism was explored by multiple spectroscopic and molecular docking methods.
- AAP can inhibit Cu/ZnSOD activity and trigger conformational changes in Cu/ZnSOD.
- AAP can spontaneously bind into the Cu/ZnSOD interface of two subdomains.