Proteomic profiling of the acid tolerance response (ATR) during the enhanced biomethanation process from Taihu Blue Algae with butyrate stress on anaerobic sludge

► Along with the enhanced anaerobic digestion process, both the activity of dehydrogenase and the protein expression were increased accordingly. ► Proteomic profiling was investigated, and proteins associated with the bioenergy metabolism were identified by MALDI-TOF MS. ► pIs of the identified proteins were found to be acidic, which strongly indicated a microbial ATR with acid stress on anaerobic sludge.