کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5788627 | 1414259 | 2017 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A simple fabricated thickness-based stiffness gradient for cell studies
ترجمه فارسی عنوان
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
چکیده انگلیسی
In this work, we developed a simple method to fabricate a thickness-based continuous stiffness gradient for biological studies. It was made by glass slides, polydimethylsiloxane (PDMS) pre-polymer, spacer and clips only, without any sophisticated equipment. It is easy to fabricate in any general biological and pharmaceutical laboratories. The stiffness gradient was characterized in terms of apparent Young's modulus by atomic force microscopy (AFM) and the Young's modulus along the gradient was found to be 8.5-120Â kPa, which is within the physiological relevant range. HeLa-C3 cells were cultured on the gradient to study their morphological behavior according to the substrate stiffness. Furthermore, the drug efficiency of etoposide, an anti-cancer drug, was studied along the substrate stiffness gradient. It was found that HeLa-C3 cells cultured on the soft region of the gradient (8.5-11Â kPa) are more sensitive to etoposide. We believe the proposed device could promote cell investigations and drug screenings on a substrate with comparable stiffness to the native tissue.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science Bulletin - Volume 62, Issue 3, 15 February 2017, Pages 222-228
Journal: Science Bulletin - Volume 62, Issue 3, 15 February 2017, Pages 222-228
نویسندگان
Yiwei Shu, Ho Nam Chan, Dongshi Guan, Hongkai Wu, Lan Ma,