کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5788779 | 1414270 | 2017 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel male-specific SET domain-containing gene setdm identified from extra microchromosomes of gibel carp males
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel male-specific SET domain-containing gene setdm identified from extra microchromosomes of gibel carp males A novel male-specific SET domain-containing gene setdm identified from extra microchromosomes of gibel carp males](/preview/png/5788779.png)
چکیده انگلیسی
Various genes have been screened on extra chromosomes, but their molecular characterization, expression pattern and biological function are still unclear. Here, we utilized a male-specific sequence of polyploid gibel carp (Carassius gibelio) to identify a novel male-specific SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain-containing gene setdm on extra microchromosomes of gibel carp males. And setdm was characterized in molecule and expression aspects, in which its expression was specific to testis, and had relative high transcription during middle/late stages of testis development. Moreover, prominent expression of Setdm in spermatogenic cells was observed in testis through immunofluorescence co-localization analysis. These results suggest that biological function of setdm might be related to testis development and spermatogenesis of gibel carp. Additionally, the homeologous gene setdmf of setdm, was also characterized, and its expression was gonad-specific, in which its expressed product was detected to mainly distribute in gametogenic cells of testis and ovary, and to have dynamic expression pattern similar to that of setdm. Based on the current results, we propose that the novel male-specific setdm on extra microchromosomes might be functional divergence gene of the gonad-specific setdmf. Therefore, these findings will help us to further understand evolutionary fate and functional role of genes on extra microchromosomes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science Bulletin - Volume 62, Issue 8, 30 April 2017, Pages 528-536
Journal: Science Bulletin - Volume 62, Issue 8, 30 April 2017, Pages 528-536
نویسندگان
Xi-Yin Li, Xiao-Li Liu, Miao Ding, Zhi Li, Li Zhou, Xiao-Juan Zhang, Jian-Fang Gui,