کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5789105 | 1414294 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reprogramming mature terminally differentiated adipocytes to induced pluripotent stem cells
ترجمه فارسی عنوان
برنامه ریزی مجدد آدیپوسیت های متمایز پایدار به سلول های بنیادی پلورپوفتون القا شده
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
چکیده انگلیسی
Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem (iPS) cells from mouse mature adipocytes by forced expression of six transcription factors (Oct4, Sox2, c-Myc, Klf4, Rarγ, and Lrh1) with a piggyBac transposon-based strategy. The resulting iPS cells were pluripotent as evidenced by the fact that they stained positive for alkaline phosphatase, expressed high levels of key pluripotency markers including Oct4, Nanog, and SSEA1, and remained pluripotent on a 2i media. In vitro differentiation of the iPS cells showed that the cell derivatives of all three germ layers could be readily obtained through formation of embryoid bodies. Most importantly, these adipocyte-derived iPS cells were capable of producing chimera with high frequencies when reintroduced into early-stage embryos and transmitted through the germ line. This study demonstrates that the new six-factor reprogramming technology facilitates the reset of the terminally differentiated adipocytes to the ground state of pluripotency, enabling us to fully explore the potential of mature adipocytes as a viable cell source for regenerative medicine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science Bulletin - Volume 60, Issue 20, October 2015, Pages 1752-1758
Journal: Science Bulletin - Volume 60, Issue 20, October 2015, Pages 1752-1758
نویسندگان
Tao Nie, Wei Deng, Xuefei Gao, Wei Sun, Xiaoyan Hui, Hong Song, Dajiang Qin, Aimin Xu, Peng Li, Pentao Liu, Liangxue Lai, Donghai Wu,