Enantiomer-specific toxicity and bioaccumulation of alpha-cypermethrin to earthworm Eisenia fetida

► (+)-(1R-cis-αS)-alpha-cypermethrin was 30 times more toxic than the (−)-isomer to earthworm. ► The chiral HPLC analysis method for alpha-cypermethrin in earthworm tissue was established. ► The bioaccumulation of alpha-cypermethrin in earthworm was enantioselective.