کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5791030 | 1554072 | 2016 | 6 صفحه PDF | دانلود رایگان |
- One SNP was found in the promoter of PGAM2 gene.
- PGAM2 gene has an association with water holding capacity.
- TT genotype could be a promising genetic markers for the selection of pork with high water holding capacity.
The phosphoglycerate mutase 2 (PGAM2) gene encodes a key enzyme in the glycolytic process. This study examined a functional mutation in the PGAM2 gene and evaluated its relationship with water holding capacity (WHC). RT-qPCR analysis showed the PGAM2 mRNA level was significantly higher in the low-WHC group than in the high-WHC group (P < 0.05). The c.-360 T > C mutation was identified through sequencing and found to have opposite allele distributions in the two groups. The allele was further genotyped in 170 Duroc Ã Large White Ã Yorkshire crossbred pigs using allele-specific PCR. The CC genotype was associated with lower WHC and higher PGAM2 mRNA levels, whereas the TT genotype corresponded to a higher WHC and lower PGAM2 mRNA levels (P < 0.05). A luciferase activity assay also showed that the CC-genotype promoter had higher activity than the TT-genotype promoter (P < 0.05). In conclusion, we discovered the c.-360 T > C mutation in the PGAM2 gene, which is a promising marker for improving pork WHC.
Journal: Meat Science - Volume 122, December 2016, Pages 139-144