کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5792580 1109640 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication
چکیده انگلیسی

A commercially available real-time PCR, based on a multi-copy target cytochrome b (cyt b) using porcine specific primers, has been validated for the Halal/Kosher authentication of gelatine. Extraction and purification of DNA from gelatine were successfully achieved using the SureFood® PREP Animal system, and real-time PCR was carried out using SureFood® Animal ID Pork Sens kit. The minimum level of adulteration that could be detected was 1.0% w/w for marshmallows and gum drops. A small survey was undertaken of processed food products such as gum drops, marshmallows and Turkish delight, believed to contain gelatine. Of fourteen food products from Germany, two samples were found to contain porcine gelatine, whereas of twenty-nine samples from Turkey twenty-eight were negative. However, one product from Turkey contained porcine DNA and thus was not Halal, and neither was the use of porcine gelatine indicated on the product label.

► Porcine DNA detection by real-time PCR in gelatine. ► Adulteration of Halal and Kosher foods. ► Survey of authenticity of gelatine-containing confectionary products.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 90, Issue 3, March 2012, Pages 686-689
نویسندگان
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