کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5794362 | 1554306 | 2016 | 11 صفحه PDF | دانلود رایگان |
- Investigation on M1 and M2 macrophages recruitment during tendon regeneration after oAECs allotransplantation in ovine
- Results evidence M1 towards M2 macrophage skew in oAEC treated tendons.
- Pro-regenerative M2 macrophages correlate with microarchitecture recovery in oAEC treated tendons.
- oAECs in vivo have an anti-inflammatory effect supporting their pro-regenerative potential.
- New insights are provided on macrophages involvement during oAECs tendon regeneration.
Recently, we have demonstrated that ovine amniotic epithelial cells (oAECs) allotransplanted into experimentally induced tendon lesions are able to stimulate tissue regeneration also by reducing leukocyte infiltration. Amongst leukocytes, macrophages (MÏ) M1 and M2 phenotype cells are known to mediate inflammatory and repairing processes, respectively. In this research it was investigated if, during tendon regeneration induced by AECs allotransplantation, M1MÏ and M2MÏ phenotype cells are recruited and differently distributed within the lesion site. Ovine AECs treated and untreated (Ctr) tendons were explanted at 7, 14, and 28Â days and tissue microarchitecture was analyzed together with the distribution and quantification of leukocytes (CD45 positive), MÏ (CD68 pan positive), and M1MÏ (CD86, and IL12b) and M2MÏ (CD206, YM1 and IL10) phenotype related markers. In oAEC transplanted tendons CD45 and CD68 positive cells were always reduced in the lesion site. At day 14, oAEC treated tendons began to recover their microarchitecture, contextually a reduction of M1MÏ markers, mainly distributed close to oAECs, and an increase of M2MÏ markers was evidenced. CD206 positive cells were distributed near the regenerating areas. At day 28 oAECs treated tendons acquired a healthy-like structure with a reduction of M2MÏ. Differently, Ctr tendons maintained a disorganized morphology throughout the experimental time and constantly showed high values of M1MÏ markers. These findings indicate that M2MÏ recruitment could be correlated to tendon regeneration induced by oAECs allotransplantation. Moreover, these results demonstrate oAECs immunomodulatory role also in vivo and support novel insights into their allogeneic use underlying the resolution of tendon fibrosis.
Journal: Research in Veterinary Science - Volume 105, April 2016, Pages 92-102