کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5794645 1554311 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Intramammary infusion of Panax ginseng extract in bovine mammary gland at cessation of milking induces changes in the expression of toll-like receptors, MyD88 and NF-kB during early involution
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Intramammary infusion of Panax ginseng extract in bovine mammary gland at cessation of milking induces changes in the expression of toll-like receptors, MyD88 and NF-kB during early involution
چکیده انگلیسی


- Bovine mammary gland responded to PG possibly by TLR2 and TLR4 signaling pathway.
- The inoculation of PG increased the expression of proinflammatory cytokines.
- The percentages of immunopositive cells for NF-kB were higher in PG-treated quarters.
- Activation of the NF-kB was verified in bovine mammary glands inoculated with PG.

The purposes of this study were to explore TLR2 and TLR4 participation and MyD88 and NF-κB activation in bovine mammary glands (BMG) treated with Panax ginseng (PG) at involution and verify the effect of PG in the cytokine expression. Quarters were infused at the end of lactation with PG solution (3 mg/ml), placebo or kept as uninoculated controls. Cows were slaughtered at 7 d after cessation of milking and mammary tissue samples were taken. A significant increase of TLR2, TLR4, MyD88, NF-κB, IL-1β, IL-6 and TGF-β1 mRNA expression was observed in PG-treated quarters. Immunostaining of TLR2 and TLR4 was significantly higher in PG mammary tissues. The percentages of immunopositive cells for NF-κB-p65 were significantly higher in PG-treated quarters. The BMG responded to PG extract components possibly by TLR2 and TLR4 signaling pathway. These results provide an insight into potential mechanisms by which PG stimulates innate immunity during BMG involution.

Graphical Abstract

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Veterinary Science - Volume 100, June 2015, Pages 52-60
نویسندگان
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