Cloning and expression of mink (Neovison vison) interferon-γ gene and development of an antiviral assay

- The mink IFN-γ cDNAs was successfully amplified by RT-PCR, predicted to be 501 bp.
- Mink is very genetically close to kinship with domestic ferret and Eurasian badger.
- Mature soluble reMiIFN-γ was expressed and had significant antiviral activity.
- Polyclonal antibodies blocked antiviral activity of MiIFN-γ.

Minks (Neovison vison) farming is under a threat of a variety of viral infections with increasingly growing number of breeding in Northeastern and Western China. While interferon is effective in controlling viral infection, IFN among different species rarely share high homology enough to provide cross protective effect on inhibition of virus replication. We cloned, sequenced, phlogenetically analyzed and expressed the miIFN-γ gene in prokaryotic and eukaryotic cells. The anti-vesicular stomatitis virus (VSV) activity of miIFN-γ protein was tested in MDCK cells using in vitro cytopathic inhibition assay. The recombinant miIFN-γ could inhibit VSV replication in MDCK cells, which was confirmed by that pre-incubation of rabbit anti-miIFN-γ antibodies with miIFN-γ abrogated the miIFN-γ protective effect. Our findings implicated that the miIFN-γ gene may be a potential counter measure against viral infection in the mink farming.