کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5794874 | 1110501 | 2013 | 7 صفحه PDF | دانلود رایگان |
An in vitro model was used to investigate effects of β-hydroxybutyrate and isoproterenol (β-adrenergic receptor agonist) on lipolysis in isolated adipocytes from late pregnant and recently calved dairy cows (n = 5) and cows with clinical ketosis (n = 3). Incubation with 3.0 mmol/L β-hydroxybutyrate reduced lipolysis in isolated adipocytes. This inhibitory effect was lower in the first lactation week (47% ± 16%) compared with late pregnancy (71% ± 6.5%). Incubation with 0.3 μmol/L isoproterenol stimulated lipolysis in isolated adipocytes from periparturient dairy cows. Basal lipolysis resulted in non-esterified fatty acid to glycerol ratios in the incubation media of 2.0 ± 0.23 in prepartum samples, 2.1 ± 0.23 in the first lactation week and 2.2 ± 0.09 in cows with clinical ketosis. β-Hydroxybutyrate reduced lipolysis by 45% ± 9.6% in isolated adipocytes from cows with clinical ketosis, indicating that impaired feedback of β-hydroxybutyrate may not play a role in the disease etiology.
Journal: Research in Veterinary Science - Volume 94, Issue 3, June 2013, Pages 433-439