کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5794879 | 1110501 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The effects of epidermal growth factor (EGF) on the in vitro development of isolated goat secondary follicles and the relative mRNA expression of EGF, EGF-R, FSH-R and P450 aromatase in cultured follicles The effects of epidermal growth factor (EGF) on the in vitro development of isolated goat secondary follicles and the relative mRNA expression of EGF, EGF-R, FSH-R and P450 aromatase in cultured follicles](/preview/png/5794879.png)
The effects of varying concentrations of EGF were evaluated in terms of in vitro follicular development and the mRNA expression levels of EGF, EGF-R, FSH-R and P450 aromatase. After 6Â days, the addition of 50Â ng/mL of EGF to the culture medium increased the antrum formation rates in comparison to cultured control and after 18Â days of culture produced oocytes with higher rates of meiosis resumption when compared to the other treatments (PÂ <Â 0.05). The daily follicular growth rates in presence of EGF (50 or 100) were increased in comparison to the cultured control (PÂ <Â 0.05). Treatment with EGF 50 stimulated the expression of EGF mRNA but reduced EGF-R mRNA expression and estradiol secretion as compared to the cultured control (PÂ <Â 0.05). After 18Â days of culture, the mRNA levels for FSH-R and P450 aromatase were greater than those of the non-cultured controls (PÂ <Â 0.05). In conclusion, the effects of EGF treatment on the mRNA levels for EGF, EGF-R, FSH-R, and P450 aromatase varied according to the stage of follicle development.
Journal: Research in Veterinary Science - Volume 94, Issue 3, June 2013, Pages 453-461