کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5795986 1110684 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Serological and coprological comparison for rapid diagnosis of Fasciola hepatica infection in small ruminants from sub-tropical area of Pakistan
ترجمه فارسی عنوان
مقایسه سرولوژیک و کاپورولوژیکی برای تشخیص سریع عفونت فاسیولا هپاتیکا در حیوانات کوچک از مناطق زیرپودر پاکستان
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

The objective of present study was to determine the prevalence of Fasciola hepatica infection in small ruminants by using indirect ELISA and sedimentation techniques and made a comparison between both techniques for rapid diagnosis. A total of 1200 serum and fecal samples from 612 sheep and 588 goats were analyzed for IgG antibodies and fecal egg count. Other parameters such as breed, age and sex were also taken into consideration. The results showed that the infection was significantly (p < 0.05) higher in sheep as compared to goats. In sheep, a prevalence of 39.2% was found using the indirect ELISA and 28.43% for the fecal analysis, while in goat the prevalence was 4.08% and 5.01%, respectively. The results showed that there was a significant (p < 0.05) difference in prevalence between breeds of sheep and goats. The results also indicated that in goat there was no significant (p > 0.05) difference in prevalence between age and sex groups. After contrasting data from ELISA and fecal analysis, 5.5% of the sera analyzed had positive values of indirect-ELISA and negative by fecal analysis. In conclusion, the findings suggest that indirect ELISA may be an efficient technique for early diagnosis of infection compared to coprological examination. The combination of both techniques was very helpful for demonstrating the current status of F. hepatica infection, and can be recommended for epidemiological surveys and for anthelmintics treatment to minimize the major health hazard affecting the production potential of small ruminants.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Small Ruminant Research - Volume 113, Issue 1, June 2013, Pages 267-272
نویسندگان
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