کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5796291 | 1554386 | 2012 | 5 صفحه PDF | دانلود رایگان |

The present study was conducted to investigate the effect of the exogenous addition of cysteamine (200 μM/ml) and epidermal growth factor (EGF; 20 ng/ml) to the maturation medium on maturation, fertilization and subsequent development of sheep oocytes, in vitro. The ovaries (n = 113) of slaughtered ewes were collected from local abattoirs in a warm normal saline solution, containing Gentamicin sulfate (50 μg/ml) in a thermos flask. The acceptable and fair quality oocytes obtained by the puncture aspiration technique were subjected to maturation in three different media viz.; for 24 h under 5% CO2 and humidified atmosphere at 38.5 °C. The matured oocytes were then subsequently subjected to fertilization using cauda epididymis spermatozoa from slaughtered rams and cultured for 6 days. The maturation percentage recorded in the EGF supplemented medium (84.9%) was significantly higher than in the cysteamine supplemented (69.6%) and control medium (67.2%). The fertilization and cleavage rates recorded were 73.7%, 49.5%, 72.3%, 52.7% and 76.5%, 55.5% in control, cysteamine and EGF supplemented media respectively. These differences were non-significant (p > 0.05). The morula formation rate recorded in the EGF supplemented (38.7%) and cysteamine supplemented (36.7%) media was significantly (p < 0.05) higher, than in the control (22.1%) medium. In conclusion, the results indicate that EGF plays an important role in the in vitro maturation and both EGF and cysteamine have a stimulatory effect on further embryonic development in sheep.
Journal: Small Ruminant Research - Volume 106, Issues 2â3, August 2012, Pages 160-164