Virus-host interactomes - antiviral drug discovery

► Experimental methods to study virus-host interactomes including mammalian validation systems. ► Yeast-two-hybrid (Y2H) as classical high-throughput method for protein-protein interaction analysis. ► Cellular antiviral drug target candidates. ► HTY2H screening identifies immunophilins (cyclophilins and FK506-binding proteins) as anticoronaviral targets. ► cyclosporine A has broad-spectrum anticoronaviral potential.