کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5814071 1556622 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cannabinoid agonists rearrange synaptic vesicles at excitatory synapses and depress motoneuron activity in vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
Cannabinoid agonists rearrange synaptic vesicles at excitatory synapses and depress motoneuron activity in vivo
چکیده انگلیسی


- Cannabinoid (CB) agonists depressed excitatory inputs on motoneurons via presynaptic CB1.
- Mechanism of action involved depletion of the readily-releasable pool of synaptic vesicles.
- Motoneuron depolarization induced CB1-dependent depression of excitatory inputs.
- Motoneurons are potential sources of CBs production “on demand”.
- A CB agonist depressed motoneuron activity in vivo presumably via CB1.

Impairment of motor skills is one of the most common acute adverse effects of cannabis. Related studies have focused mainly on psychomotor alterations, and little is known about the direct impact of cannabinoids (CBs) on motoneuron physiology. As key modulators of synaptic function, CBs regulate multiple neuronal functions and behaviors. Presynaptic CB1 mediates synaptic strength depression by inhibiting neurotransmitter release, via a poorly understood mechanism. The present study examined the effect of CB agonists on excitatory synaptic inputs incoming to hypoglossal motoneurons (HMNs) in vitro and in vivo. The endocannabinoid anandamide (AEA) and the synthetic CB agonist WIN 55,212-2 rapidly and reversibly induced short-term depression (STD) of glutamatergic synapses on motoneurons by a presynaptic mechanism. Presynaptic effects were fully reversed by the CB1-selective antagonist AM281. Electrophysiological and electron microscopy analysis showed that WIN 55,212-2 reduced the number of synaptic vesicles (SVs) docked to active zones in excitatory boutons. Given that AM281 fully abolished depolarization-induced depression of excitation, motoneurons can be feasible sources of CBs, which in turn act as retrograde messengers regulating synaptic function. Finally, microiontophoretic application of the CB agonist O-2545 reversibly depressed, presumably via CB1, glutamatergic inspiratory-related activity of HMNs in vivo. Therefore, evidence support that CBs, via presynaptic CB1, induce excitatory STD by reducing the readily releasable pool of SVs at excitatory synapses, then attenuating motoneuron activity. These outcomes contribute a possible mechanistic basis for cannabis-associated motor performance disturbances such as ataxia, dysarthria and dyscoordination.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 92, May 2015, Pages 69-79
نویسندگان
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