The α3β4* nicotinic acetylcholine receptor subtype mediates nicotine reward and physical nicotine withdrawal signs independently of the α5 subunit in the mouse

► AuIB, a selective α3β4 antagonist, was used to assess α3β4 in nicotine behaviors. ► α3β4 nicotinic receptors do not mediate acute nicotine behaviors. ► α3β4 nicotinic receptors mediate nicotine reward and withdrawal independent of α5. ► α3β4 nicotinic receptors are involved in somatic, but not affective withdrawal.