MDMA produces a delayed and sustained increase in the extracellular concentration of glutamate in the rat hippocampus

► MDMA produces a delayed, sustained increase in extracellular glutamate in the hippocampus. ► Treatment with fluoxetine or ketanserin attenuates MDMA-induced glutamate efflux. ► The increase in extracellular glutamate produced by MDMA is not diminished in the presence of TTX.