Carisbamate (RWJ-333369) inhibits glutamate transmission in the granule cell of the dentate gyrus

► We investigated the synaptic mechanism of carisbamate in the dentate gyrus. ► Carisbamate inhibited glutamatergic transmission. ► GABAergic transmission and neuronal excitability were not affected. ► The antiepileptic effect of carisbamate is from inhibiting presynaptic Na channels.