Sumatriptan inhibition of N-type calcium channel mediated signaling in dural CGRP terminal fibres

► The 5-HT1 receptor agonist sumatriptan is an effective migraine therapeutic. ► The antimigraine anatomical locus and mechanisms of action remain unresolved. ► We show sumatriptan acts peripherally at individual dural CGRP pain fibre terminals. ► N-type Ca2+ channels selectively mediate sumatriptan inhibition of Ca2+ signaling. ► Findings provide insight for developing novel peripherally targeted therapeutics.