کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5827393 | 1558926 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Magnolol and honokiol regulate the calcium-activated potassium channels signaling pathway in Enterotoxigenic Escherichia coli-induced diarrhea mice
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کلمات کلیدی
IP3PKCCaMKIIαMagnolol (PubChem CID: 72300)enterotoxigenic Escherichia coli - Esterichia coli Enterotoxigenicinositol 1,4,5-trisphosphate - inositol 1،4،5-trisphosphateDiarrhea - اسهالCAM - ساخت به کمک کامپیوترMagnolol - مگنولولHonokiol - هونکیوولProtein kinase C - پروتئین کیناز سیCalcium-activated potassium channel - کانال پتاسیم فعال کلسیمInositol 1,4,5-trisphosphate receptor - گیرنده inositol 1،4،5-trisphosphate
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب سلولی و مولکولی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To explore the regulatory mechanisms of magnolol and honokiol on calcium-activated potassium channels signaling pathway in Enterotoxigenic Escherichia coli (ETEC)-induced diarrhea mice, the concentrations of serum chloride ion (Clâ), sodium ion (Na+), potassium ion (K+) and calcium ion (Ca2+) were measured. Additionally, the mRNA expressions of calmodulin 1 (CaM), calcium/calmodulin-dependent protein kinase II alpha subunit (CaMKIIα) and beta subunit (CaMKIIβ), ryanodine receptor 1, inositol 1,4,5-trisphosphate receptors (IP3 receptors), protein kinases C (PKC), potassium intermediate/small conductance calcium-activated channels (SK) and potassium large conductance calcium-activated channels(BK)were determined. A diarrhea mouse model was established using ETEC suspensions (3.29Ã109 CFU/ml) at a dosage of 0.02 ml/g live body weight (BW). Magnolol or honokiol was intragastrically administered at dosages of 100 (M100 or H100), 300 (M300 or H300) and 500 (M500 or H500) mg/kg BW according to a 3Ã3 factorial arrangement. Magnolol and honokiol increased the Clâ and K+ concentrations, further, upregulated the CaM, BKα1 and BKβ3 mRNA levels but downregulated the IP3 receptors 1, PKC, SK1, SK2, SK3, SK4 and BKβ4 mRNA expressions. Magnolol and honokiol did not alter the CaMKIIα, CaMKIIβ, ryanodine receptor 1, IP3 receptor 2, IP3 receptor 3, BKβ1 and BKβ2 mRNA expressions. These results clarify that magnolol and honokiol, acting through Ca2+ channel blockade, inhibit the activation of IP3 receptor 1 to regulate the IP3-Ca2+ store release, activate CaM to inhibit SK channels, and effectively suppress PKC kinases to promote BKα1 and BKβ3 channels opening and BKβ4 channel closing, which modulates the intestinal ion secretion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmacology - Volume 755, 15 May 2015, Pages 66-73
Journal: European Journal of Pharmacology - Volume 755, 15 May 2015, Pages 66-73
نویسندگان
Yanli Deng, Xuefeng Han, Shaoxun Tang, Wenjun Xiao, Zhiliang Tan, Chuanshe Zhou, Min Wang, Jinghe Kang,