کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
583053 877865 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study on the protective role of selenium against cadmium toxicity in lactic acid bacteria: An advanced application of ICP-MS
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بهداشت و امنیت شیمی
پیش نمایش صفحه اول مقاله
Study on the protective role of selenium against cadmium toxicity in lactic acid bacteria: An advanced application of ICP-MS
چکیده انگلیسی
In this work, Lactobacillus casei rhamnosus were obtained from the commercial product of fermented milk and possible antagonistic effect of selenium (as sodium selenite) against cadmium toxicity was studied. The bacteria capability to incorporate Se was demonstrated: after 1 week exposure to Se(IV), its total concentration in the freeze-dried biomass was 405 ± 28 μg/g (7.4 ± 0.8 μg/g in control). In the presence of Se(IV) and Cd(II), the bacterial growth and cell viability were improved and lipid peroxidation less marked with respect to bacteria exposed to Cd(II) alone. The distribution of Se and Cd in molecular mass fractions of bacteria extracts was investigated by size exclusion chromatography with diode array and ICP-MS detection. The results obtained suggest that the antagonistic effect of Se is due to lower incorporation of cadmium at a high molecular mass (MM < 600 kDa). Slightly different distribution of elements in the fractions of MM < 40 kDa suggests the formation of new chemical species involving Cd and Se in bacteria exposed to Cd(II) + Se(IV) as compared to those exposed to Cd(II) alone. The study illustrates the high utility of atomic spectrometry to critically inform molecular questions that could be important in the industrial processes based on bacterial activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Hazardous Materials - Volume 153, Issue 3, 30 May 2008, Pages 1157-1164
نویسندگان
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