Effects of some drugs on hepatic glucose 6-phosphate dehydrogenase activity in Lake Van Fish (Chalcalburnus Tarischii Pallas, 1811)

IC50 values of vankomycine, sulfanylamide, sulfanylacetamide, nidazole, ciprofloxacin, amoxicillin and KMnO4 were 1.88, 0.037, 0.032, 1.178, 2.26, 643.5 and 0.0002 mM, and the Ki constants 1.18 ± 0.148, 0.119 ± 0.021, 0.075 ± 0.015, 1.15 ± 0.21, 7.69 ± 0.67, 1007 ± 69, and 0.001 ± 0.00022 mM, respectively. While vankomycine and nidazole showed competitive inhibition, others displayed noncompetitive inhibition. Ki constants and IC50 values for drugs were determined by Lineweaver-Burk graphs and plotting activity percentage versus [I], respectively.