کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5848618 1561699 2016 28 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of combined exposure to formaldehyde and benzene on immune cells in the blood and spleen in Balb/c mice
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Effects of combined exposure to formaldehyde and benzene on immune cells in the blood and spleen in Balb/c mice
چکیده انگلیسی
Formaldehyde and benzene are the two major indoor air pollutants due to their prevalence and toxicity. This study aimed to explore the toxic effect on the spleen and relevant immune responses of Balb/c mice caused by exposure to a combination of formaldehyde and benzene. Balb/c mice were divided randomly into five groups (n = 9/group): blank control group (Ctrl); solvent ([corn] Oil) control; formaldehyde only (FA, 3 mg/m3); benzene only (BZ, 150 mg/kg BW); and, formaldehyde + benzene group (FA + BZ). Exposures were performed for 8 h/day, 5 day/week, for 2 weeks. Tail blood was collected after the final exposure; 24-h later, the mice were euthanized to permit assessment of a variety of immune endpoints. The endpoints' three areas were: (1) in living mice, body weight and delayed-type hypersensitivity (DTH) responses; (2) in blood, immune cell counts and serum antibody levels (serum hemagglutination); and, (3) in spleen samples, reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), caspase-3 (cell apoptosis) levels and lymphocyte proliferation. In this study we fund (1) BZ and FA + BZ exposure can lead to the reduction in the number of some immune cells in peripheral blood; (2) Formaldehyde has certain synergistic effects on benzene-induced cytotoxicity in peripheral blood, (3) FA, BZ and FA + BZ exposure can lead to ROS and GSH depletion in spleen cells, and spleen cell apoptosis (caspase-3 increased) may be one of the downstream events, decreased splenic lymphocyte proliferation; and (4) the FA + BZ combined exposure can lead to the decreased body weight, serum antibody level (by serum hemagglutination assay).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Toxicology and Pharmacology - Volume 45, July 2016, Pages 265-273
نویسندگان
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