کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5848628 | 1561699 | 2016 | 6 صفحه PDF | دانلود رایگان |
- AE-COS inhibited activation of MMP-2 and MMP-9.
- AE-COS exerted inhibitory effect on MMP-9 expression.
- AE-COS reduced activation of NF-κB.
- AE-COS inhibited invasion of human fibrosarcoma cells.
Chitooligosaccharides (COS) have been reported to show a variety of biological efficacies such as anti-bacterial activity, anti-tumor activity and immune activity. The purpose of this study is to investigate the inhibitory effect of aminoethyl-chitooligosaccharides (AE-COS) synthesized from COS that were substituted hydroxyl groups with aminoethyl group at C-6 position on cell invasion of human fibrosarcoma cells. First of all, the effect of AE-COS on cell viability was observed using MTT assay. The cytotoxicity of AE-COS was increased in a dose dependent manner. The inhibitory effects of AE-COS on the activity and expression level of MMP-2 and MMP-9 related to invasion of cancer cells were examined using gelatin zymography and western blot. It was found that AE-COS above 20 μg/ml showed the inhibitory effect on the activity and expression of MMP-9. Furthermore, AE-COS at 20 μg/ml reduced the expression level of p50, a part of NF-κB, compared with phorbol-12- myristate-13- acetate (PMA) group. The available data let us hypothesize that AE-COS could provide chemoprevention as an inhibitor against cell invasion associated with metastasis.
Journal: Environmental Toxicology and Pharmacology - Volume 45, July 2016, Pages 309-314