Application of the yeast-based reporter gene bioassay for the assessment of estrogenic activity in cow's milk from Poland

- The estrogenic potential of Polish cow's milk was evaluated by the yeast estrogen bioassay.
- Significant estrogenic activity was detected in 50% of milk samples after deconjugation.
- Deconjugation is an obligatory step to reveal overall estrogenic activity of milk.
- Positive correlation was found between fat content and estrogenic activity.
- Human dietary intake of estrogens with milk may be higher than serum estrogen levels.