Short communicationInfluence of the presence of ethanol on in vitro bioavailability of fungicide residues

- The influence of the ethanol content on the dialysis capacity of the fungicides is appreciated.
- No significant differences in bioavailability in wines of 11, 13 and 14.5% ethanol, were found for any of the fungicides.
- Differences exist between the bioavailability and the presence of ethanol, but no direct relationship with increasing in alcohol content.
- An increase in the degree of alcohol increases the percentage dialyzed for ametoctradin, mepanipyrim, cyazofamid, pyraclostrobin and metrafenona, and decreases the dialyzación of dimethomorph, boscalid and kresoxim-methyl.

The influence of ethanol on the in vitro bioavailability of nine fungicides in wines of varying degrees of alcohol is studied by simulating the digestive process by dialysis in semipermeable membranes. The dose of each fungicide corresponds to its added Maximum Residue Limits in the different matrices (water, ethanol, wines of 7, 11, 13 and 14.5% ethanol). A validated analytical methodology was used which includes extraction, partition according to the modified QuEChERS multiresidue method and liquid chromatography with triple quadrupole tandem mass spectrometry analysis. Interaction between the ethanol content and bioavailability was confirmed and increases were found in the dialyzed percentages with respect to the blank (the standard in water) for ametoctradin, mepanipyrim, cyazofamid, pyraclostrobin and metrafenone, while a decrease was observed for dimethomorph, boscalid and kresoxim-methyl. Fenhexamid showed no significant differences by alcohol content.