کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5849330 | 1561752 | 2016 | 44 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An experimental platform using human intestinal epithelial cell lines to differentiate between hazardous and non-hazardous proteins
ترجمه فارسی عنوان
یک پلتفرم تجربی با استفاده از خطوط سلولی اپیتلیال روده ای انسان برای جداسازی پروتئین های خطرناک و غیر خطرناک
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کلمات کلیدی
سموم پروتئین، سلولهای اپیتلیال روده، سمیت مسمومیت،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
چکیده انگلیسی
Human intestinal epithelial cell lines (T84, Caco-2, and HCT-8) grown on permeable Transwell⢠filters serve as models of the gastrointestinal barrier. In this study, this in vitro model system was evaluated for effectiveness at distinguishing between hazardous and non-hazardous proteins. Indicators of cytotoxicity (LDH release, MTT conversion), monolayer barrier integrity ([3H]-inulin flux, horseradish peroxidase flux, trans-epithelial electrical resistance [TEER]), and inflammation (IL-8, IL-6 release) were monitored following exposure to hazardous or non-hazardous proteins. The hazardous proteins examined include streptolysin O (from Streptococcus pyogenes), Clostridium difficile Toxins A and B, heat-labile toxin from enterotoxigenic Escherichia coli, listeriolysin O (from Listeria monocytogenes), melittin (from bee venom), and mastoparan (from wasp venom). Non-hazardous proteins included bovine and porcine serum albumin, bovine fibronectin, and ribulose bisphosphate carboxylase/oxygenase (RuBisco) from spinach. Food allergenic proteins bovine milk β-lactoglobulin and peanut Ara h 2 were also tested as was the anti-nutritive food protein wheat germ agglutinin. Results demonstrated that this model system effectively distinguished between hazardous and non-hazardous proteins through combined analysis of multiple cells lines and assays. This experimental strategy may represent a useful adjunct to multi-component analysis of proteins with unknown hazard profiles.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 92, June 2016, Pages 75-87
Journal: Food and Chemical Toxicology - Volume 92, June 2016, Pages 75-87
نویسندگان
Bryan P. Hurley, Waheed Pirzai, Alex D. Eaton, Marc Harper, Jason Roper, Cindi Zimmermann, Gregory S. Ladics, Raymond J. Layton, Bryan Delaney,