کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5850143 | 1561774 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chimaphilin induces apoptosis in human breast cancer MCF-7 cells through a ROS-mediated mitochondrial pathway
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کلمات کلیدی
MMPDPPHMCF-7DCFH-DANAC2′,7′-dichlorofluorescin diacetate - 2 '، 7'-dichlorofluorescin diacetate3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyl tetrazolium bromideMTT - MTTROS - ROSAcridine orange - آکاردین نارنجیApoptosis - خزان یاختهایhuman breast cancer cell line - سلول سرطان سینه انسانMCF-7 cells - سلول های MCF-7Mitochondrial - میتوکندریاMitochondrial membrane potential - پتانسیل غشای میتوکندریPropidium iodide - پروتئین یدیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Chimaphilin, 2,7-dimethyl-1,4-naphthoquinone, is extracted from pyrola [Passiflora incarnata Fisch.]. In this study, the anticancer activity and underlying mechanisms of chimaphilin toward human breast cancer MCF-7 cells are firstly investigated. Chimaphilin could inhibit the viability of MCF-7 cells in a concentration-dependent manner, and the IC50 value was 43.30 μM for 24 h. Chimaphilin markedly induced apoptosis through the investigation of characteristic apoptotic morphological changes, nuclear DNA fragmentation, annexin V-FITC/propidium iodide (PI) double staining. Flow cytometry assay revealed that chimaphilin triggered a significant generation of ROS and disruption of mitochondrial membrane potential. Additionally, western blotting assay showed that chimaphilin suppressed Bcl-2 level and enhanced Bad level, then activated caspase-9 and caspase-3, and further activated the poly ADP-ribose polymerase (PARP), finally induced cell apoptosis involving the mitochondrial pathway. Furthermore, free radical scavengers N-acetyl-L-cysteine (NAC) pretreatment test testified that chimaphilin could increase the generation of ROS, then induce cell apoptosis. In general, the present results demonstrated that chimaphilin induced apoptosis in human breast cancer MCF-7 cells via a ROS-mediated mitochondrial pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 70, August 2014, Pages 1-8
Journal: Food and Chemical Toxicology - Volume 70, August 2014, Pages 1-8
نویسندگان
Wei-Dong Ma, Yong-Peng Zou, Peng Wang, Xiao-Hui Yao, Yao Sun, Ming-Hui Duan, Yu-Jie Fu, Bo Yu,