کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5850787 | 1561778 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The non-cytotoxicity characterization of rebaudioside A as a food additive
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The non-cytotoxicity characterization of rebaudioside A as a food additive The non-cytotoxicity characterization of rebaudioside A as a food additive](/preview/png/5850787.png)
چکیده انگلیسی
To evaluate the cytotoxicity of high-purity rebaudioside A (reb A, 99.16%) as a food ingredient, a combination of several methods, including tetrazolium-based colorimetric assay (MTT), lactate dehydrogenase assay (LDH), enzyme-linked immunosorbent assay (ELISA), real-time PCR (qPCR), high-performance liquid chromatography (HPLC), and two-dimensional electrophoresis (2-DE) were used to test the cytotoxicity of reb A on the human cells HT-29 and T84, as well as liver and spleen cells from mice. The results indicated that no obvious changes in cellular viability, inflammatory cytokines yield, or protein yield were observed between the test group and the control group when different concentrations of reb A were used, suggesting that reb A is non-cytotoxic in vitro at the concentrations range tested (0.001-0.5%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 66, April 2014, Pages 334-340
Journal: Food and Chemical Toxicology - Volume 66, April 2014, Pages 334-340
نویسندگان
Xiaoli Wu, Baogui Wang, Tingtao Chen, Min Gan, Xingxing Chen, Fei Chen, Hua Wei, Feng Xu,