کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5851224 | 1561788 | 2013 | 7 صفحه PDF | دانلود رایگان |
The α subunit of β-conglycinin is a major allergen in soybean. The objective of this study was to predict and identify the linear immunoglobulin (Ig)E epitopes of the soybean α subunit of β-conglycinin. Three immunoinformatics tools were used to predict the potential epitopes and were confirmed by dot-blot inhibition using sera from soybean allergic subjects. As a result, 15 peptides were predicted and assembled by solid-phase synthesis. Eleven epitopes were identified by the dot-blot inhibition test. Moreover, peptide 3 had IgE binding capability with all sera(5/5) tested, while peptide 1, 4, 6, 8 and12 could bind to 4/5 of the sera samples. Secondary structure prediction of peptide 3 and circular dichroism test validated that the structure of peptide 3 was a random coil.
⺠Three immunoinformatics tools were used to predict the potential epitopes. ⺠Dot-blot inhibition using sera from soybean allergic subjects confirm the epitopes. ⺠11/15 of the predicted epitopes can react with 3/5 human sera. ⺠All the predicted epitopes can react with at least one of the human sera. ⺠One of epitopes can react with all of human sera.
Journal: Food and Chemical Toxicology - Volume 56, June 2013, Pages 254-260