کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5851389 | 1561791 | 2013 | 8 صفحه PDF | دانلود رایگان |

Ganodermanondiol, a biologically active compound, was isolated from the Lingzhi mushroom (Ganoderma lucidum). The present study examined the protective effects of ganodermanondiol against tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity. Ganodermanondiol protected human liver-derived HepG2 cells through nuclear factor-E2-related factor 2 (Nrf2) pathway-dependent heme oxygenase-1 expressions. Moreover, ganodermanondiol increased cellular glutathione levels and the expression of the glutamine-cysteine ligase gene in a dose-dependent manner. Furthermore, ganodermanondiol exposure enhanced the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and its upstream kinase activators, LKB1 and Ca2+/calmodulin-dependent protein kinase-II (CaMKII). This study indicates that ganodermanondiol exhibits potent cytoprotective effects on t-BHP-induced hepatotoxicity in human liver-derived HepG2 cells, presumably through Nrf2-mediated antioxidant enzymes and AMPK.
Highlights⺠Ganodermanondiol was isolated from the Lingzhi mushroom (Ganoderma lucidum). ⺠Ganodermanondiol exhibits potent cytoprotective effects on t-BHP-induced hepatotoxicity in human liver-derived HepG2 cells. ⺠Ganodermanondiol showed potent cytoprotective effects through Nrf2 pathway-dependent expression of HO-1 and GSH. ⺠Ganodermanondiol exposure enhanced the phosphorylation of AMPK and its upstream kinase activators, LKB1 and CaMKIIα.
Journal: Food and Chemical Toxicology - Volume 53, March 2013, Pages 317-324