Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species

The methanol and aqueous extracts of three endemic Centaurea species (C. polypodiifolia var. pseudobehen, C. pyrrhoblephara and C. antalyense) were investigated for their antioxidant and cholinesterase inhibitory activities. The antioxidant activities of these extracts were evaluated by in vitro models including, phosphomolybdenum assay, free radical scavenging assays (DPPH and ABTS), β-carotene/linoleic acid test system, metal chelating assay, FRAP assay, ferric and cupric reducing power. Cholinesterase inhibitory activities were examined using Ellman's colorimetric method. Total phenol, flavonoid, and saponin contents were also measured. Among the six Centaurea extracts evaluated, the highest antioxidant abilities were obtained from C. polypodiifolia var. pseudobehen. Methanolic extracts from C. polypodiifolia var. pseudobehen and C. antalyense had a noticeable inhibition towards AChE and BChE. These findings suggest that Centaurea species could be an anticholinesterase agent and antioxidant resource in some industries, such as food, pharmacology, and cosmetics.

► We reported antioxidant and anticholinesterase properties of three Centaurea species. ► Aqueous and methanolic extracts of the Centaurea species were tested. ► The studied Centaurea extracts exhibited strong antioxidant activity. ► Anticholinesterase activities of the species were assessed for the first time. ► The species under study are a valuable source of antioxidants and AChE inhibitors.