کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5851960 1561793 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Promoting effect of polysaccharide isolated from Mori fructus on dendritic cell maturation
ترجمه فارسی عنوان
ترویج اثر پلی ساکارید جدا شده از موری فروتوس بر بلوغ سلول دندریتیک
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی

Maturation of dendritic cells (DCs) is usually attenuated in the tumor microenvironment, which is an important immunological problem in DC-based immunotherapy of cancer. In this study, we report the effect of a Mori fructus polysaccharide (MFP) on DC maturation. MFP was treated to DCs generated from mouse BM cells. MFP induced phenotypic maturation of DCs, as proven by the increased expression of CD40, CD80/86, and MHC-I/II molecules. MFP induced functional maturation of DCs, in that MFP increased the expression of IL-12, IL-1β, TNF-α, and IFN-β, decreased antigen capture capacity, and enhanced allogenic T cell stimulation. MFP efficiently induced maturation of DCs from C3H/HeN mice having normal toll-like receptor4 (TLR4), but not DCs from C3H/HeJ mice having mutated TLR4, suggesting that TLR4 might be one of the membrane receptors of MFP. As a mechanism of action, MFP increased phosphorylation of mitogen-activated protein kinase (MAPKs), and nuclear translocation of NF-κB p65 subunit, which were important signal molecules downstream from TLR4. These data suggest that MFP induces DC maturation through TLR4 and MFP can be used as an adjuvant in DC-based cancer immunotherapy.

► MFP isolated from Mori fructus increases the expression of MHC-I/II and costimulatory molecules in DCs. ► MFP increases the production of cytokines, decreased antigen uptake, and enhanced allogenic T cell stimulation of DCs. ► MFP promotes DC maturation via MAPKs and NF-κB signalings downstream of TLR4.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 51, January 2013, Pages 411-418
نویسندگان
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