کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5852115 | 1561787 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Melanoidins isolated from heated potato fiber (Potex) affect human colon cancer cells growth via modulation of cell cycle and proliferation regulatory proteins
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کلمات کلیدی
high molecular weight fractionNF-κβHMWCDKLMWERKIC503-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromidec-Raf - C-RafMTT - MTTSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAAcridine orange - آکاردین نارنجیColon cancer - سرطان کولونnuclear factor kappa-light-chain-enhancer of activated B cells - فاکتور هسته ای kappa-light-chain-enhancer از سلول های B فعال شده استAntiproliferative activity - فعالیت ضد انعقادیMEK - مجاهدین خلقMelanoidins - ملانوئیدی هاCell cycle inhibition - مهار سلول سلولیhalf-maximal inhibitory concentration - نیمه حداکثر غلظت مهاریretinoblastoma protein - پروتئین رتینوبلاستوماmitogen-activated protein kinase kinase - پروتئین کیناز کیناز فعال Mitogen فعالPropidium iodide - پروتئین یدیدLow molecular weight fraction - کسر وزن مولکولی کمextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیcyclin-dependent kinase - کییناز وابسته به سیکلین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Melanoidins are brown, nitrogen containing, high molecular weight end products of Maillard reaction with poorly established activity towards tumor cells. The goal of present study was to verify whether both heated potato fiber Potex extract (180 °C for 2 h) and melanoidins isolated from the extract exerts growth-inhibiting activity in human colon cancer cells in vitro. The cells of LS180 colon cancer cell line were tested upon treatment with roasted potato fiber extract (AM4) as well as with high (HMW) and low (LMW) molecular weight fractions isolated from the extract, since both may be regarded as/or contain melanoidins. The tested compounds at concentration of 1000 μg/ml reduced cell growth down to 45%, 69% and 54%, respectively. Furthermore, deregulated ERK1/2 signaling was revealed upon treatment. Moreover, multiple alternations in cell cycle regulators activity were found (i.e. cyclinD1, cyclin-dependent kinase 4 and 6, p21, p27, p53, pRb) leading to cell cycle cessation in G0 phase. Importantly, LMW compounds revealed markedly stronger potential to alter specific molecular targets comparing to HMW compounds. Summarizing, the results emphasize that both high and low molecular weight melanoidins contribute to antiproliferative activity of heated potato fiber in LS180 colon cancer cells in vitro.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 57, July 2013, Pages 246-255
Journal: Food and Chemical Toxicology - Volume 57, July 2013, Pages 246-255
نویسندگان
Ewa Langner, Fernando M. Nunes, Piotr Pożarowski, Martyna Kandefer-SzerszeÅ, Stefan G. Pierzynowski, Wojciech Rzeski,