کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5852213 | 1130848 | 2012 | 9 صفحه PDF | دانلود رایگان |

The aim of the present study was to evaluate the protective effect and possible mechanism of the total flavonoids (TFs) from Rosa Laevigata Michx fruit (RLMF) against hydrogen peroxide (H2O2) induced damage in human umbilical vein endothelial cells (HUVECs). The cell injury caused by H2O2 was protected by pretreatment with the TFs for 1 h. Compared with the model group, the TFs decreased S phase cells, suppressed nuclear morphological damage, inhibited the collapse of mitochondrial membrane potentials (ÎΨm), attenuated excessive reactive oxygen species generation, reduced glutathione depletion, impacted the mitochondrial morphology change, decreased caspase-3, -9 activities, and decreased fragmented DNA. Further mechanism investigation showed that the TFs could increase the protein expressions of Procaspase-3, Bcl-2, and decrease the expressions of Bak, Bax, Bid and p53. Generally, the TFs from RLMF is an effective natural product for the treatment of cardiovascular and cerebrovascular diseases.
⺠TFs from RLMF could protect HUVECs against H2O2 induced damage. ⺠TFs could keep the normal morphology of nuclear and mitochondria. ⺠TFs had antioxidant activity. ⺠TFs could affect the expressions of some proteins related to mitochondrial pathway.
Journal: Food and Chemical Toxicology - Volume 50, Issue 9, September 2012, Pages 3133-3141