Safety assessment of mushroom β-glucan: Subchronic toxicity in rodents and mutagenicity studies

► Mushroom β-glucan was investigated for subchronic toxicity and mutagenicity. ► In the subchronic study, no adverse effects of mushroom β-glucan were noted at 2000 mg/kg/day. ► In mutagenicity studies, no genotoxic effects of mushroom β-glucan were noted. ► The no-observed-adverse-effect level (NOAEL) for mushroom β-glucan was 2000 mg/kg/day.