کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5853874 | 1130868 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Codonopsis lanceolata extract induces G0/G1 arrest and apoptosis in human colon tumor HT-29 cells - Involvement of ROS generation and polyamine depletion
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کلمات کلیدی
5-FUCodonopsis lanceolataJnkN-acetyl-l-cysteineRT-PCRNACc-Jun N-terminal kinase - C-Jun N-terminal kinaseROS - ROSApoptosis - خزان یاختهایROS generation - نسل ROSreverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسHigh pressure liquid chromatography - کروماتوگرافی مایع با فشار بالاHPLC - کروماتوگرافی مایعی کاراn-butanol fraction - کسر نان بوتانولGlu - گلوGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش تغذیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Codonopsis lanceolata (Campanulasea) is widely distributed and grown in Asia and has been in use as traditional medicine for long time. The n-butanol fraction (BF) of C. lanceolata significantly inhibited human colon cancer HT-29 cell growth in a dose- and time-dependent manner by inducing G0/G1 phase arrest and apoptosis. The inhibition was associated with intracellular ROS generation and polyamine depletion as evidenced by HPLC quantitatively. Additionally, semi-quantitative RT-PCR revealed enhanced expression of caspase-3, p53, and the Bax/Bcl-2 ratio and reduced expression of survivin in HT-29 cells treated with BF. Furthermore, western blot analysis of p53, JNK, and caspase-3 showed that ROS generation was accompanied by JNK activation. Increase of the Bax/Bcl-2 ratio and activation of caspase-3 might be due to intracellular polyamine depletion. Conclusively, the findings of this study imply a critical role of ROS and polyamine depletion in the anticancer effects of C. lanceolata root extract.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food and Chemical Toxicology - Volume 49, Issue 1, January 2011, Pages 149-154
Journal: Food and Chemical Toxicology - Volume 49, Issue 1, January 2011, Pages 149-154
نویسندگان
Lan Wang, Ming Lu Xu, Jian He Hu, Søren K. Rasmussen, Myeong-Hyeon Wang,