کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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5873694 | 1144644 | 2015 | 10 صفحه PDF | دانلود رایگان |

BackgroundThe aim of the present study was to investigate changes in the expression of CD163 and hemoglobin oxygenase-1 (HO-1) in brain tissue surrounding hematomas after intracerebral hemorrhage (ICH), and correlations with other factors.Materials and methodsBrain tissues in the close surrounding of ICH hematomas (nâ=â27, ICH group) were collected at 6âhours or less, 6-24âhours, 24-72âhours, and more than 72âhours after bleeding onset, and more distant tissues (nâ=â12, control group) were histologically analyzed with hematoxylin and eosin staining and transmission electron microscopy. Interleukin (IL)-1, IL-10, and tumor necrosis factor-alpha, as well as the expression of CD163 and HO-1, were assessed using immunochemistry, Western blotting, and reverse transcription-polymerase chain reaction. Apoptosis rates were determined with terminal deoxynucleotidyl transferase dUTP nick end labeling assays.ResultsThe expressions of the inflammatory cytokines IL-1 and tumor necrosis factor-alpha were increased at 6-24âhours (Pâ<.05), reached a peak at 24-72âhours (Pâ<.001 and Pâ<.01), at which time histopathological changes became most obvious and apoptosis rates were highest, but diminished for more than 72âhours after ICH onset. The anti-inflammatory cytokine IL-10 peaked at 6-24âhours (Pâ<â.01) after ICH onset but dropped in the following periods to lower levels than the control (Pâ<.05). CD163 and HO-1 expressions gradually increased from 6 to 24âhours to peaks at more than 72âhours after ICH onset (Pâ<.001).ConclusionThe highest inflammation level in tissues surrounding ICH hematomas occurred 2-3 days after bleeding onset, but was accompanied by an anti-inflammatory factor IL-10 expression enhancement. In the period of more than 72âhours after ICH onset, CD163 and HO-1 expressions reached peaks and inflammatory cytokine expressions dropped.
Journal: Journal of Stroke and Cerebrovascular Diseases - Volume 24, Issue 12, December 2015, Pages 2800-2809