Nef interaction with actin compromises human podocyte actin cytoskeletal integrity

► Podocyte proteins interacting with Nef were identified. ► Nef expressing podocytes displayed scant number of actin filament. ► Nef enhanced the expression of Rac1, syndecan-4, Rif, and CDC42. ► Interaction between Nef and actin altered podocyte phenotype.