کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5888244 1152309 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Serum miR-210 as a novel biomarker for molecular diagnosis of clear cell renal cell carcinoma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی بالینی
پیش نمایش صفحه اول مقاله
Serum miR-210 as a novel biomarker for molecular diagnosis of clear cell renal cell carcinoma
چکیده انگلیسی

ObjectiveOur objective was to evaluate the levels of miR-210 in tumor and serum samples of conventional renal cell cancer (cRCC) patients to explore whether circulating miR-210 in serum can be used as a biomarker for the detection of cRCC.MethodsThe paired samples from primary cRCC tumors and adjacent non-tumoral renal parenchyma were collected from 32 patients with cRCC. Serum samples were obtained from 68 patients with a cRCC before surgery, 10 samples after one week of surgery, and 42 healthy individuals were included in this study. Real-time PCR was used to measure the microRNA level. The expression of miRNAs was normalized using the dCT method. Expression levels of miR-210 were compared using the Mann-Whitney U test or Wilcoxon test. Diagnostic performance of serum miR-210 level was calculated by using the receiver operating characteristic (ROC) curve.ResultsThe average miR-210 level was higher in primary cRCC tissues than in normal tissue (p = 0.004). For serum samples, the average level of miR-210 was significantly higher in cRCC patients than in controls (p < 0.001). The serum miR-210 level yielded an AUC (the areas under the ROC curve) of 0.874 with a sensitivity of 81.0% and a specificity of 79.4%. Furthermore, the average serum level of miR-210 was significantly decreased in the patients one week after the operation (p = 0.001).ConclusionSerum mi-210 may have a potential as a novel noninvasive biomarker for the detection of cRCC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental and Molecular Pathology - Volume 94, Issue 1, February 2013, Pages 115-120
نویسندگان
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