کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5897600 1155272 2013 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alternative splicing and proteolytic rupture contribute to the generation of soluble IL-6 receptors (sIL-6R) in rheumatoid arthritis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Alternative splicing and proteolytic rupture contribute to the generation of soluble IL-6 receptors (sIL-6R) in rheumatoid arthritis
چکیده انگلیسی

ObjectiveTo describe the relationship between the two mechanisms involved in sIL6R generation in rheumatoid arthritis (RA).MethodRA patients were selected from a group of subjects genotyped for the rs8192284 SNP, located at the proteolytic cleavage site of IL-6R. sIL6R and protease levels (ADAM17) were measured and the contribution of alternative splicing in the generation of sIL-6R was evaluated through qRT-PCR.ResultIncreased sIL-6R plasma levels and expression of spliced isoform generating sIL-6R are genotype dependent. ADAM17 concentrations were independent of the genotype studied.ConclusionAlternative splicing and proteolytic cleavage participate in sIL-6R generation in RA. The rs8192284 polymorphism determines the sIL-6R plasma level through differential proteolytic rupture controlled by ADAM17.

► Two processes of sIL-6R generation coexist in RA. ► The genetic background affects the proteolytic generation of sIL-6R. ► These data could be important for a better application of therapies targeting sIL6R.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 61, Issue 3, March 2013, Pages 720-723
نویسندگان
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