Molecular characterization of prostaglandin F receptor (FP) and E receptor subtype 3 (EP3) in chickens

► Chicken prostaglandin E receptor subtype 3 (cEP3) & F receptor (cFP) were cloned. ► An alternative splice variant cFPb was first identified in chicken. ► cEP3 and cFP were widely expressed in adult chicken tissues. ► Chicken EP3 primarily couples to Gi-cAMP pathway, but may also cause MAPK activation. ► Chicken FP primarily couples to Ca2+ pathway, but may also cause MAPK activation.