کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905142 | 1159847 | 2016 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Research paperDirect and indirect effects of RNA interference against pyridoxal kinase and pyridoxine 5â²-phosphate oxidase genes in Bombyx mori Research paperDirect and indirect effects of RNA interference against pyridoxal kinase and pyridoxine 5â²-phosphate oxidase genes in Bombyx mori](/preview/png/5905142.png)
- Application of RNA interference against PLK and PNPO genes in Bombyx mori
- Expression of PLK and PNPO has a greater impact on transcription of PSAT and GOT.
- PLP biosynthesis and dynamic balance may be regulated by genetic networks.
Vitamin B6 comprises six interconvertible pyridine compounds (vitamers), among which pyridoxal 5â²-phosphate is a coenzyme involved in a high diversity of biochemical reactions. Humans and animals obtain B6 vitamers from diet, and synthesize pyridoxal 5â²-phosphate by pyridoxal kinase and pyridoxine 5â²-phosphate oxidase. Currently, little is known on how pyridoxal 5â²-phosphate biosynthesis is regulated, and pyridoxal 5â²-phosphate is supplied to meet their requirement in terms of cofactor. Bombyx mori is a large silk-secreting insect, in which protein metabolism is most active, and the vitamin B6 demand is high. In this study, we successfully down-regulated the gene expression of pyridoxal kinase and pyridoxine 5â²-phosphate oxidase by body cavity injection of synthesized double-stranded small interfering RNA to 5th instar larvae of Bombyx mori, and analyzed the gene transcription levels of pyridoxal 5â²-phosphate dependent enzymes, phosphoserine aminotransferase and glutamic-oxaloacetic transaminase. Results show that the gene expression of pyridoxal kinase and pyridoxine 5â²-phosphate oxidase has a greater impact on the gene transcription of enzymes using pyridoxal 5â²-phosphate as a cofactor in Bombyx mori. Our study suggests that pyridoxal 5â²-phosphate biosynthesis and dynamic balance may be regulated by genetic networks.
Journal: Gene - Volume 587, Issue 1, 1 August 2016, Pages 48-52