کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905195 | 1159857 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inducible expression of indoleamine 2,3-dioxygenase attenuates acute rejection of tissue-engineered lung allografts in rats
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کلمات کلیدی
SAGMECMPBSTNFαHprtTGF-β1MSCIDOAECIFNγH&E - H & EMOI - MEELT - آموزش زبان انگلیسیIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیindoleamine 2,3-dioxygenase - ایندولامین 2،3-دیوکسژیگنازCOPD - بیماری مزمن انسدادی ریهChronic obstructive pulmonary disease - بیماری مزمن انسدادی ریهtransforming growth factor beta 1 - تبدیل فاکتور رشد بتا 1regulated on activation, normal T cell expressed and secreted - تنظیم شده در فعال سازی، سلول T طبیعی بیان و ترشح می شودtumor necrosis factor alpha - تومور نکروز عامل آلفاImmunosuppression - سرکوب سیستم ایمنیMesenchymal stem cell - سلول های بنیادی مزانشیمیalveolar epithelial cells - سلولهای اپیتلیال آلوئولارExtracellular matrix - ماتریکس خارج سلولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریRANTES - مطالبHematoxylin & Eosin - هماتوکسیلین و ائوزینLung transplantation - پیوند ریهmultiplicity of infection - چندین عفونتInterferon gamma - گاما اینترفرون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Lung disease remains one of the principal causes of death worldwide and the incidence of pulmonary diseases is increasing. Complexity in treatments and shortage of donors leads us to develop new ways for lung disease treatment. One promising strategy is preparing engineered lung for transplantation. In this context, employing new immunosuppression strategies which suppresses immune system locally rather than systemic improves transplant survival. This tends to reduce the difficulties in transplant rejection and the systemic impact of the use of immunosuppressive drugs which causes side effects such as serious infections and malignancies. In our study examining the immunosuppressive effects of IDO expression, we produced rat lung tissues with the help of decellularized tissue, differentiating medium and rat mesenchymal stem cells. Transduction of these cells by IDO expressing lentiviruses provided inducible and local expression of this gene. To examine immunosuppressive properties of IDO expression by these tissues, we transplanted these allografts into rats and, subsequently, evaluated cytokine expression and histopathological properties. Expression of inflammatory cytokines IFNγ and TNFα were significantly downregulated in IDO expressing allograft. Moreover, acute rejection score of this experimental group was also lower comparing other two groups and mRNA levels of FOXP3, a regulatory T cell marker, upregulated in IDO expressing group. However, infiltrating lymphocyte counting did not show significant difference between groups. This study demonstrates that IDO gene transfer into engineered lung allograft tissues significantly attenuates acute allograft damage suggesting local therapy with IDO as a strategy to reduce the need for systemic immunosuppression and, thereby, its side effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 576, Issue 1, Part 3, 15 January 2016, Pages 412-420
Journal: Gene - Volume 576, Issue 1, Part 3, 15 January 2016, Pages 412-420
نویسندگان
Ammar Ebrahimi, Gholam Ali Kardar, LadanTeimoori Toolabi, Hossein Ghanbari, Esmaeil Sadroddiny,