کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905391 | 1159882 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for the conservation of miR-223 in zebrafish (Danio rerio): Implications for function
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کلمات کلیدی
HaematopoiesisqPCRDPFMiRNA targetshpfOsteoclastogenesis - استئوکلستوژنزmiR-223 - به miR-223miRNA expression - بیان miRNArapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدbase pairs - جفت پایهkilobase pairs - جفت کیلوباdays post-fertilization - روز پس از لقاحReal time-PCR - زمان واقعی PCRConservation - زیستشناسی بقاhours post-fertilization - ساعت پس از لقاحRace - مسابقهUTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNANucleotide - نوکلئوتید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
MicroRNAs (miRNAs) are an abundant and conserved class of small RNAs, which play important regulatory functions by interacting with the 3â² untranslated region (UTR) of target mRNAs. Through this mechanism, miR-223 was shown to regulate genes involved in mammalian haematopoiesis, both in physiological and pathological contexts. MiR-223 is essential for normal myelopoiesis in mammals, promoting granulocyte, osteoclast and megakaryocyte differentiation and suppressing erythropoiesis. However, there is a general lack of knowledge regarding miR-223 function in other vertebrates, which could help to clarify its role in other processes, such as development. In this work, we explored the functional conservation of miR-223 using zebrafish as a model. We show that miR-223 gene structure and genomic context have been maintained between human and zebrafish. In addition, we identified 22 novel sequences of miR-223 precursor and demonstrate that it contains domains highly conserved among vertebrates, suggesting function preservation throughout evolution. Furthermore, collected evidences show that miR-223 expression is highly correlated with haematopoietic events and osteoclastogenesis throughout zebrafish development. In adults, expression of miR-223 in zebrafish tissues mimics the distribution in mice, with high levels found in the major fish haematopoietic organ, the head kidney. These results suggest a conservation of miR-223 role in haematopoiesis, and osteoclastogenesis between zebrafish and human. Accordingly, validated targets of miR-223 in mammalian models were investigated and defined as putative targets in zebrafish, by in silico and gene expression analysis. Our data compiles critical evidence showing that miR-223, a highly conserved miRNA, appears to have kept similar regulatory functions throughout evolution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 566, Issue 1, 15 July 2015, Pages 54-62
Journal: Gene - Volume 566, Issue 1, 15 July 2015, Pages 54-62
نویسندگان
V.P. Roberto, D.M. Tiago, K. Gautvik, M.L. Cancela,