کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5905540 | 1159908 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning, characterization, and expression of sheep FGF5 gene
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کلمات کلیدی
DMEMORFcomplete coding sequenceFGF5rapid amplification of the cDNA endsUTRDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccoamino acid - آمینو اسیدgenetic variants - انواع ژنتیکیopen reading frame - قاب خواندن بازRace - مسابقهuntranslated region - منطقه غیر ترجمهhemagglutinin epitope - هموگلوتینینSingle-nucleotide polymorphism - پلی مورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتیدCdS - کادمیم سولفید، سولفید کادمیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
The fibroblast growth factor 5 gene (FGF5) is a member of the FGF gene family, and represents a candidate gene for hair length because of its role in the regulation of the hair follicle growth cycle. In our current study, we cloned, sequenced, and characterized the full-length FGF5 cDNA of Chinese Merino sheep. We obtained the complete genomic sequence of the FGF5 gene from sheep blood samples, and compared it to other FGF5 sequences in GenBank. We found that the FGF5 gene spanned 21,743Â bp of genomic DNA, and consisted of 3 exons and 2 introns, both of which differed from those of a previously annotated FGF5 genomic sequence from sheep. We also identified a previously undescribed FGF5 mRNA splicing variant, FGF5S, and the western blot analysis showed that the molecular weights of the FGF5 (34Â kDa) and FGF5s (17Â kDa) proteins were consistent with the estimates based on the genomic and cDNA sequence data. We examined the expression of both FGF5 mRNAs in various tissues of sheep, and found that the expression of the FGF5S mRNA was restricted to the brain, spleen, and skin tissue. The single-nucleotide polymorphism analysis of the genomic sequence revealed 72 genetic variants of the FGF5 gene. Our findings provide insight into the functions of the FGF5 gene in Chinese Merino.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 95-100
Journal: Gene - Volume 555, Issue 2, 25 January 2015, Pages 95-100
نویسندگان
Lihua Zhang, Sangang He, Mingjun Liu, Guosong Liu, Zheng Yuan, Chenxi Liu, Xumei Zhang, Ning Zhang, Wenrong Li,